Guanidine Thiocyanate CAS 593-84-0

Product Description:

Product Name: Guanidine thiocyanate CAS NO: 593-84-0

Synonyms:

Thiocyanic acid, compd. with guanidine (1:1);

thiocyanic acid,guanidine salt;

guanidine monothiocyanate;

Chemical & Physical Properties:

Appearance: White powder

Assay :≥99.0%

Density: 1.103 g/mL at 20ºC

Boiling Point: 132.9℃ at 760 mmHg

Melting Point: 118-122℃

Flash Point: 34.2℃

Refractive Index: n20/D 1.482

Stability: Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents.

Storage Condition: Store at RT.

Safety Information:

RTECS: XL1225000

Hazard Class: 8

Safety Statements: S13-S61

HS Code: 2925290090

Packing Group: III

WGK Germany: 2

RIDADR: UN 1759

Risk Statements: R20/21/22; R32; R52/53

Hazard Code: Xn

Symbol: GHS05, GHS07

Caution Statement: P260; P280; P301 + P312 + P330; P303 + P361 + P353; P304 + P340 + P310; P305 + P351 + P338

Supplemental Hazard Statements: Contact with acids liberates very toxic gas.

Hazard Declaration: H302 + H312 + H332; H314; H412

Signal Word: Danger

Guanidinium thiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used in the extraction of DNA and RNA.

Note: this compound may also be recognized as guanidine thiocyanate. This is because guanidinium is the conjugate acid of guanidine and is called the guanidinium cation, [CH6N3]+.

Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 "Spanish flu", so that it can be studied safely. This application is presumably based on its denaturation activity.

Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them. These enzymes would otherwise damage the extract.

A commonly used method is guanidinium thiocyanate-phenol-chloroform extraction. It is not strictly necessary to use phenol or chloroform if extracting RNA for Northern blotting or DNA for Southern blot analysis because the gel electrophoresis followed by transfer to a membrane will separate the RNA/DNA from the proteins. Additionally, since these methods use probes to bind to their conjugates, peptides that get through the process don't generally matter unless a peptide is an RNase or DNase, and then only if the enzyme manages to renature, which should not occur if proper protocols are followed. A possible exception might be when working with temperature extremophiles because some enzymes of these organisms can remain stable under extraordinary circumstances.

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