Tris(Hydroxymethyl)Aminomethane CAS 77-86-1 Buffer Chemical C4H11NO3

Product Description:

Product Name: Tris(hydroxymethyl)aminomethane CAS 77-86-1

Synonyms:

TRIS; Tris Base;

Tromethamine;

Chemical & Physical Properties:

Appearance: White crystalline power

Assay :≥99.0%

Density: 1.35g/cm3

Melting point:167-172℃

Boiling Point:357℃ at 760 mmHg

Flash Point:219-220℃

Application:

Tris and its derivatives are commonly used in the preparation of buffer solutions and the production of drugs in biological experiments, especially in protein and nucleic acid related experiments where Tris is required as a biological buffer.

Tris Base is also used as an intermediate for biochemical reagents and fosfomycin, as well as a vulcanization promoter, cosmetics (creams, lotions), mineral oil, paraffin emulsifier, and biological buffer.

Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in e ther and carbon tetrachloride, corrosive to copper and aluminum.

Buffering characteristics:

Tris is a weak base, with a pKa of 8.1 at 25 ℃; According to buffering theory, the effective buffering range of Tris buffer is between pH 7.0 and 9.2. The pH of Tris alkali aqueous solution is around 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain a buffer solution with that pH value. However, attention should also be paid to the effect of temperature on the pKa of Tris.

Advantage:

1. Tris is a weak base, and using this buffer system alone can prepare a buffer solution with a wide range of pH values from acidic to alkaline;

2. There is minimal interference with the experimental results and no precipitation occurs with calcium, magnesium ions, and heavy metal ions.

PH range

At room temperature (25 ℃), the effective buffering range of the buffer solution is between pH 7.0 and 9.2.

Generally, hydrochloric acid/sodium hydroxide is added to adjust the pH value to the desired value, while paying attention to the influence of temperature.

Tris buffer solution: pH=7.5~9.0;

Tris HCl buffer solution: pH=7.5~8.5;

Tris phosphate buffer solution: pH=5.0~9.0.

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